6、 Agrisera 植物一抗来源物种对WB实验是否有影响？是否起重要作用？
7、如有 Agrisera 植物一抗 可以应用于 immunolocalization是否也能应用于WB？
8、 如有 Agrisera 植物一抗 可以 适 用于拟南芥， 是否也能适用 于其他物种？
9 、 如某一 Agrisera 植物一 抗 产品 说明书 中 未提到 客户待 检测物种， 应该如何判断此一抗是否能适用于此待检测物种 ？
1 0 、 客户 购买 Agrisera抗体，使用后实验结果不理想，怎么办 ？
1 1 、 客户 如何选择 合适的 Agrisera 植物 抗体？
12
、
Agrisera
未上
架
的抗体申请试用
样品
，需要客户提供哪些信息？
1、 Agrisera 的抗体 是否可以提供试用样品 ？
在售产品不提供 试用样品。 未上架产品需要客户提供非常详细的实验样品信息后 由Agrisera厂家 评估发放，而且 需要 客户在收到试用样品后三个月内 提供 实验结果 。
2、 Agrisera 等待上架产品预计供货时间？
由于植物抗体验证需要各类样本，突变体对照等，样品准备周期很长，所以 导致 一般植物抗 体验证周期非常长， 1-2 年时间都会出现 。
4、 Agrisera 抗体在血清的浓度是多少？
大部分情况， 特异性抗体在血清的浓度在 0.05-0.2mg/ml 之间。特殊情况下，表达强的多抗血清中包含有 1-3mg/ml 的特异性抗体，甚至更多。
5、 应该如何保存 Agrisera 抗体？ 能保存多久？
抗体尽量避免反复冻融，收到抗体后根据实验需要对抗体进行分装。严格按照说明书推荐的保存条件正确处理和保存抗体。
Each antibody is different, some might last for 20-40 years, while other antibodies will have quite a short life of one year. Storage conditions are of importance to extend antibody life, however the stability of each specific antibody might be different. Therefore, please follow the information provided on the specific product information sheet for each antibody. What works for one antibody, might not necessarily be applicable for another one. This is very important to keep in mind. If it is not clear ， please contact us . General recommendations for antibody storage can be found here .
6、 Agrisera 植物一抗来源物种对WB实验是否有影响？是否起重要作用？
It does not matter from which species the primary antibody comes from for a western blot application. There are many good secondary antibodies available for any of those species you inquire about.
What is important is to which part of a protein an antibody is made to. Is it going to recognize epitopes (stretches of 2-3 amino acids) which are exposed on a given protein after transfer to a membrane? Some proteins can refold following
the transfer, depending upon applied conditions, which can make a given epitope not accessible. It also depends on
if the western blot is performed in native or denatured conditions. The addition of 6-8 M urea to your loading buffer might help to keep your protein unfolded and accessible for an antibody to bind.
Before you use an antibody in western blot, please check to which part of
the protein it was made. Are there any references which confirms
the use of this antibody for this specific technique?
7、 如有 Agrisera 植物一抗 可以应用于 immunolocalization是否也能应用于WB ？
不一定
Again, it depends upon the antibody binding place on the protein. Is this part exposed after fixation or following the western blot? If a polyclonal antibody is made to a synthetic peptide, it is recognizing a pool of epitopes (linear epitopes are streches of 2-3 amino acids) and if such epitopes are not exposed following fixation: the antibody is not going to work.
8、 如有 Agrisera 植物一抗 可以 适 用于拟南芥， 是否也能适用 于其他物种 ？
不一定，具体 适用 物种请查看产品说明书。
Not really, it all depends upon the conservation level of a specific peptide used to elicit this antibody. It can be checked by comparing the peptide used to elicit this antibody and your protein sequence. The c onservation level between the peptide used to make an antibody and the peptide found in your protein seqence needs to be around 80 %, to allow an antibody to work in case of anti-peptide antibodies and can be lower for antibodies made to larger parts of a protein. In some cases 6-8 M urea needs to be included to fully unfold the protein. The electrophoretic mobility of a protein can also vary between different species.
9 、 如某一 Agrisera 植物一 抗 产品 说明书 中 未提到 客户待 检测物种， 应该如何判断此一抗是否能适用于此待检测物种 ？
需要客户提供待检测物种的 特定 蛋白序列， Agrisera 厂家根据 客户 提供的蛋白序列进行蛋 白比对，评估是否可以 适 用于待检测物种 。
1 0 、 客户 购买 Agrisera抗体，使用后实验结果不理想，怎么办 ？
需要客户提供具体实验信息 ，填写使用反馈表 ， Agrisera 厂家会根据 客户反馈情况 提供 具体 优化方案。
Have you checked recommended conditions to use for this specific antibody, included on the product info sheet? Some antibodies will work reasonably well and give good results regardless of the applied conditions, like membrane type, load per well and developing reagent. Some antibodies will actually require a bit more consideration. Less backgrond can be obtained for some antibodies when using pvdf vs. nitrocellulose membrane for transfer or by applying recommended blocking. There are also variations between secondary antibodies. At least, but the most important, is the type of sample you are analyzing. You can either contact us or check our Western blot trouble shooting, it can be found here .
1 1 、 客户 如何选择 合适的 Agrisera 植物 抗体？
Usually the C or N-terminal of the protein is used, as those parts of protein are exposed. Also, to mimic protein behavior, the synthesized peptide should have similar structure and charge as the protein it has been "cut out off". Therefore:
- peptides derived from the C terminal should have N terminal modified by acetylation
- peptides derived from the N terminal should have C terminal modified by amidation - peptides derived from an internal sequence should have both ends modified
Following points should also be considered:
- Are there any other proteins from the family of interest, where cross-reactivity should be avoided?
- Is the crystal structure of the protein (or homologous protein) known? This would be helpful for the peptide chemist in searching for the best peptide for antibody production.
- What is the final application of the produced antibodies? Native or denatured techniques?
12 、 Agrisera 未上 架 的抗体申请试用 样品 ，需要客户提供哪些信息？
检测物种、实验类型、蛋白序列，客户 能否承诺 收到试用 样品 后 三个月内 给予实验反馈。 Agrisera 厂家 根据客户提供的信息评估发放试用样品 。
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